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BE AWARE: This avoids the coupling of amino acids within the additional cycles with the membrane instead of the growing peptide chain. Ensure that all of the purification buffers are at four °C and perform the protein purification within the chilly room to keep away from protein denaturation. BE AWARE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and likewise some of the amino teams throughout the spot areas don't kind bonds with the amino acids.
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